Observation Data For Our Products
The field trial data demonstrate the excellent efficacy of DuroKea products over a long period which is implicated in your protection from old and emerging pathogens.
DuroKea S
Objective: To determine the antimicrobial efficacy of DuroKea products
Method:
The antimicrobial activity of Durokea S was assessed on frequently used surfaces such as lift, door handles and clinic etc. for a period of 35 days. Respective surfaces were clean from dust and DuroKea S applied. DuroKea S was sprayed different surface using standard spray machines producing fine mist and were allowed to complete dry (DuroKea S was sprayed at night 9 pm and allow to dry overnight. Till the entire duration of experiment, no other decontaminators were used on those surfaces. Sample collection days and dates have been mentioned in Tables 1 and 2. For the lift, samples were collected from the lift buttons, sides and the handle. Button outside the lift served as control where nothing was sprayed. For the clinic, samples were collected from the nurse station (chair handle and table) and visitors’ chair. The entrance door handle served as the control without any spray.
The samples from respective places were collected using sterile swabs and were then dabbed on Luria Bertani (LB) agar plates. The plates were incubated in 37°C for 12-16 h. Microbial colony formation was observed after the incubation time.
Table 1: Sample Collection From Lift
| Sample collection day | Date |
|---|---|
| Day 2 | December 5, 2020 |
| Day 5 | December 8, 2020 |
| Day 11 | December 14, 2020 |
| Day 15 | December 18, 2020 |
| Day 21 | December 24, 2020 |
| Day 30 | January 2, 2021 |
Table 2: Sample Collection From Clinic
| Sample collection day | Date |
|---|---|
| Day 2 | December 5, 2020 |
| Day 7 | December 10, 2020 |
| Day 15 | December 18, 2020 |
| Day 30 | January 2, 2021 |
Figure:
DuroKea S sample collection points (a) Clinic entrance at Indian Institute of Technology, Hyderabad (Control Surface) (b) The nurse station and (c) Visitor’s chair in the clinic where DuroKea S was applied (d) Buttons outside the lift without DuroKea S application (Control) (e) Lift surfaces where DuroKea S was applied and buttons inside the lift (inset).
Figure:
Representative images of bacterial agar plates used for the field trial (a) Lift surfaces and (b) clinic surfaces sample collection points to evaluate the long-term antimicrobial activity of DuroKea S. Samples collected from different points are denoted numerically while the non-coated surface, labelled C, served as positive control.
Observation:
DuroKea S proves to be a strong disinfectant as minimal growth of microbes was observed in both lift and clinic even after 30 days of spraying DuroKea S. The long lasting efficacy of the surface disinfectant is demonstrated by the inhibition of microbial colonies on the agar plates from samples collected over the same period. The nano formulation coating provided robust antibacterial efficacy in our field trial.
DuroKea H
Objective: To determine the antimicrobial efficacy of DuroKea products
Method: Application Method: DuroKea H, the alcohol based hand sanitizer, was sprayed on both sides of the hands of volunteers and was allowed to dry without rubbing. The volunteers were divided in two groups of 4 h and 8 h, based on the time point at which the samples were collected. The volunteers were allowed to do their regular work but were asked not to wash their hands. After the respective time points, the samples were collected from their hands using sterile swabs. The swabs were dabbed on LB agar plates and incubated for 12-16 h. The plates were then checked for microbial growth.
Sample collection points from applied hands for DuroKea H
Figure:
Bacterial culture plates showing the anti-microbial efficacy of DuroKea H at 4 and 8 h of different volunteers
Observation:
The alcohol based sanitizer was sprayed on the hands of the volunteers and samples collected after 4 and 8 h. The absence of microbial growth (no colonies) from swabs dabbed from DuroKea H applied hands shows that DuroKea H completely subdued the growth of microbes. As observed from the colonies in non-DuroKea H applied hands, the applied hand surfaces of the volunteers were completely free of microbes.
DuroKea H Aqua
Objective: To determine the antimicrobial efficacy of DuroKea products
Method Four puffs of DuroKea H Aqua, the water based hand sanitizer was applied on the hands of the volunteers. The sanitizer was properly rubbed on the hands and in between fingers until dried. Similar to DuroKea H, the volunteers of DuroKea H Aqua were divided into two groups of 4 h and 8 h. The volunteers were asked to do their regular work but not to wash their hands off. Samples were collected at the respective time points using sterile swabs and were dabbed on LB agar plates. The plates were incubated for 12-16 h and then checked for microbial growth.
Sample collection points from applied hands for DuroKea H Aqua.
Figure:
Bacterial culture plates showing the anti-microbial efficacy of DuroKea H Aqua at 4 and 8 h of different volunteers.
Observation:
The effectiveness of the aqua-based foam sanitizer was demonstrated on the volunteers (8-10) for 4 and 8 h. 90-95% killing of microbes was observed after spraying DuroKea H Aqua as observed by the absence of microbial colonies on the LB agar plates.